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AdmaXAdMin
- Posts : 555
Join date : 2016-01-05
Age : 47 
Life Sciences
Sat Apr 02, 2016 10:22 am
Generic.
Biology Biophysics Biotechnology Physiology Anatomy Biochemistry Genetics NeuroScience
- AdmaXAdMin
- Posts : 555
Join date : 2016-01-05
Age : 47
Quantitative Biology
Sun Apr 10, 2016 6:50 pm
Quantitative Biologyspecific papers :
http://arxiv.org/archive/q-bio
Genomics, recent
Neurons and Cognition , recent
Manhattan GandhiLeveL V
- Posts : 565
Join date : 2016-01-09
Age : 47
Location : R'Lyeh
Physiology Anatomy Biochemistry
Mon Feb 27, 2017 12:00 pm
Library / 
:
Guyton , Arthur - Textbook of Medical Physiology Berne & Levy - Physiology - 6E, Koeppen, Stanton Color atlas of biochemistry - Koolman, Roehm, 2E Ganong's Review of Medical Physiology -23E, 2010 Lippincott's Illustrated Reviews ,Immunology, 2013 Essential Neuroscience, 3rd Edition - Siegel , Allan Gray's Atlas of Anatomy - Vogl , Mitchell & Drake Gray's Anatomy For Students - Vogl Mitchell Drake Mark's Basic Medical Biochemistry (Clinical), 2013 Harper's Illustrated Biochemistry 30E,McGraw-Hill Sherris' Medical Microbiology , K.J.Ryan , C.G.Ray
DelearthLeveL V- Posts : 541
Join date : 2016-01-06
Age : 47
Location : Antarctica
Re: Life Sciences
Wed Aug 02, 2017 9:00 am
Complex cellular logic computation using ribocomputing devices
Nature (2017)
Synthetic biology aims to develop engineering-driven approaches to the programming of cellular functions that could yield transformative technologies1. Synthetic gene circuits that combine DNA, protein, and RNA components have demonstrated a range of functions such as bistability2, oscillation3, 4, feedback5, 6, and logic capabilities7, 8, 9, 10, 11, 12, 13, 14, 15. However, it remains challenging to scale up these circuits owing to the limited number of designable, orthogonal, high-performance parts, the empirical and often tedious composition rules, and the requirements for substantial resources for encoding and operation. Here, we report a strategy for constructing RNA-only nanodevices to evaluate complex logic in living cells. Our ‘ribocomputing’ systems are composed of de-novo-designed parts and operate through predictable and designable base-pairing rules, allowing the effective in silico design of computing devices with prescribed configurations and functions in complex cellular environments. These devices operate at the post-transcriptional level and use an extended RNA transcript to co-localize all circuit sensing, computation, signal transduction, and output elements in the same self-assembled molecular complex, which reduces diffusion-mediated signal losses, lowers metabolic cost, and improves circuit reliability. We demonstrate that ribocomputing devices in Escherichia coli can evaluate two-input logic with a dynamic range up to 900-fold and scale them to four-input AND, six-input OR, and a complex 12-input expression(A1 AND A2 AND NOT A1*) OR (B1 AND B2 AND NOT B2*) OR (C1 AND C2) OR (D1 AND D2) OR (E1 AND E2). Successful operation of ribocomputing devices based on programmable RNA interactions suggests that systems employing the same design principles could be implemented in other host organisms or in extracellular settings.
http://www.nature.com/nature/journal/vaop/ncurrent/full/nature23271.html?foxtrotcallback=true
Nature (2017)
Synthetic biology aims to develop engineering-driven approaches to the programming of cellular functions that could yield transformative technologies1. Synthetic gene circuits that combine DNA, protein, and RNA components have demonstrated a range of functions such as bistability2, oscillation3, 4, feedback5, 6, and logic capabilities7, 8, 9, 10, 11, 12, 13, 14, 15. However, it remains challenging to scale up these circuits owing to the limited number of designable, orthogonal, high-performance parts, the empirical and often tedious composition rules, and the requirements for substantial resources for encoding and operation. Here, we report a strategy for constructing RNA-only nanodevices to evaluate complex logic in living cells. Our ‘ribocomputing’ systems are composed of de-novo-designed parts and operate through predictable and designable base-pairing rules, allowing the effective in silico design of computing devices with prescribed configurations and functions in complex cellular environments. These devices operate at the post-transcriptional level and use an extended RNA transcript to co-localize all circuit sensing, computation, signal transduction, and output elements in the same self-assembled molecular complex, which reduces diffusion-mediated signal losses, lowers metabolic cost, and improves circuit reliability. We demonstrate that ribocomputing devices in Escherichia coli can evaluate two-input logic with a dynamic range up to 900-fold and scale them to four-input AND, six-input OR, and a complex 12-input expression(A1 AND A2 AND NOT A1*) OR (B1 AND B2 AND NOT B2*) OR (C1 AND C2) OR (D1 AND D2) OR (E1 AND E2). Successful operation of ribocomputing devices based on programmable RNA interactions suggests that systems employing the same design principles could be implemented in other host organisms or in extracellular settings.
http://www.nature.com/nature/journal/vaop/ncurrent/full/nature23271.html?foxtrotcallback=true
- DelearthLeveL V
- Posts : 541
Join date : 2016-01-06
Age : 47
Location : Antarctica
Re: Life Sciences
Thu Aug 04, 2022 11:00 pm
Cellular recovery after prolonged warm ischaemia of the whole body[After cessation of blood flow or similar ischaemic exposures, deleterious molecular cascades commence in mammalian cells,
eventually leading to their death. Yet with targeted interventions, these processes can be reversed, even minutes
or hours post mortem. []
After 1 h of warm ischaemia, OrganEx application preserved tissue integrity, decreased cell death and restored selected
molecular and cellular processes across multiple vital organs.]
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